What are the steps for Diff Quik in the correct order?

• A. Smear, air dry, fixative, solution 1, solution 2, water rinse
• B. Smear, fixative, air dry, solution 1, water rinse, solution 2
• C. Smear, air dry, fixative, solution 2, solution 1, water rinse
• D. Air dry, smear, fixative, solution 1, solution 2, water rinse

Answer: (A)

The steps rely on a quick, three-step Romanowsky-type staining process where preserving morphology and getting distinct colors from different cell components is key. First, you need the smear to be dry so the cells stay intact and firmly attached when you apply fixative. Allowing an air-dry phase sets the slide for proper fixation. Then apply the fixative, typically methanol, to immobilize and preserve cellular structures. After fixation, dip the slide in the first stain (Solution 1), which begins coloring the cytoplasm and other components. Next, use the second stain (Solution 2) to provide contrast, especially to nuclei and basophilic elements. Finally, rinse with water to remove excess dye and stop the staining, yielding the characteristic Diff-Quik appearance with pink-to-orange cytoplasm and blue nuclei.